Summary
Type: SPE
Application #: 304590
Catalog#: 730426 - SPE Cartridges, Chromabond, SB, 6ml, PP, 500mg, 30/pk
Detection:
Conditions: Column conditioning: 5 mL methanol, then 5 mL methanol – water (70:30, v/v), 1 mL/min (Do not let run the column dry.)
Sample aspiration: 10 mL of pretreated sample
Washing: 5 µL methanol – water (70:30, v/v), then 3 mL methanol
Elution: 10 µL acetic acid – methanol (1:99, v/v), 1 mL/min
Evaporation: to dryness (50 °C, slight stream of nitrogen)
Reconstitution: after a storage (4 °C) with 200 µL acetonitrile – water
Authors: Kritzinger, Q. et al.
Source: Department of Botany, University of Pretoria, Sout